Plasmid DNA purification using pre-packed anion-exchange resin columns to purify plasmid DNA from 200-1600 ml of cultured bacterial cells. Kit includes TrueBlue Lysis Buffer.
The GeneIsokit™ Plasmid Maxi Kit was designed for plasmid DNA purification using pre-packed anion-exchange resin maxiprep columns to purify plasmid DNA from 200-1600 ml of cultured bacterial cells. TrueBlue Lysis Buffer (an optional color indicator) is included with the kit to prevent common handling errors, while ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound to the column and the contaminants are removed with PW Buffer. The purified plasmid DNA is eluted by a high salt buffer then precipitated with isopropanol for desalting. The entire procedure can be completed in 2.5 hours without ultracentrifuges, HPLC or other toxic reagents and the purified plasmid DNA is suitable for Transfection, Sequencing Reactions, Ligation, PCR, and In-vitro Transcription, Microinjection, Restriction Enzyme Digestion and Gene Gun.
Features and Benefits
- High Yield: 1.5 mg of pure transfection grade plasmid DNA from 400 ml of cultured bacterial cells
- 200-1600 ml of cultured bacterial cells
- Storage: dry at room temperature (15-25ºC) for up to 2 years,PM1 (RNase A added) 2-8ºC for up to 6 months
- In-vitro Transcription
- Restriction Enzyme Digestion
- Gene Gun